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KMID : 0361020010440020124
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Korean Journal of Otolaryngology - Head and Neck Surgery
2001 Volume.44 No. 2 p.124 ~ p.128
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Compositional Difference of Middle Ear Effusion according to Viscosity of the Effusion
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최재영/Jae Young Choi
윤주헌/이원상/정명현/Joo Heon Yoon/Won Sang Lee/Myung Hyun Chung
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Abstract
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Background and Objectives : Serous otitis media (SOM) is usually responsive to medical treatment, whereas mucoid otitis media (MOM) is not This study was undertaken to elucidate the compositional difference between serous and mucoid effusion, and
to
investigate whether MUC5AC acts as a major mucin in the middle ear mucosa with MOM. Materials and Methods : Middle ear effusion (MEE) samples were collected from 29 patients with MOM and 20 patients with SOM. The level of mucin, lysozyme,
secretory
IgA
and interleukin-8 were measured by dot blotting or enzyme-linked immunosorbent assay. Periodic acid-Schiff and immunohistochemical staining with monoclonal anti-MUC5AC antibody were performed on the serial sections of middle ear mucosa with MOM.
Results
: Mucoid effusions contained higher levels of mucin, lysozyme, secretory IgA and interleukin-8 than serous effusion. Immunohistological study revealed that MUC5AC mucin was expressed in only a small portion of the goblet cells of middle ear
mucosa
with
MOM. Conclusion : Our study suggests that both serous secretions and mucin might make the middle ear effusion more viscous, and that mucins other than MUC5AC might have a major role in the viscosity of MEE. Further study is necessary to identify
the
major mucins in the MEE of otitis media with effusion.
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